Rhizosphere fungal community assembly varied across functional guilds in a temperate forest

Abstract Background Rhizosphere fungi play an important role in plant community dynamics and biogeochemical cycling. While the drivers of fungal assembly have been studied varied ecosystems, it is still unclear how these processes function for rhizosphere soil temperate forests. Furthermore, unknown whether relative contributions determinants remain consistent or vary across ecological guilds. This study used high-throughput next-generation sequencing to characterize communities 247 samples from 19 tree species a forest within Northeast China. We aimed investigate three forests (host species, neighbouring communities, edaphic properties) influence functional guilds trees. Results found that host contributed more pathogens’ composition than ectomycorrhizal fungi, pathogens consistently showed higher specialization fungi. Saprotrophs also high specialization, which was mediated by species’ effect on pH. Although neighboring remarkably richness all guilds, this with stronger biotrophic (plant fungi) non-biotrophic guild (saprotrophs). Neighboring shaped strongly regardless trees’ mycorrhizal type, whereas properties were most only ectomycorrhizal-associated Edaphic played shaping saprotrophic compositions, indicating importance associated absorption decomposition nutrients. Conclusions These results demonstrated reflecting their different functions ecosystems.